Nader Sheibani, PhD

Professor
Ophthalmology and Visual Sciences
1111 Highland Avenue
9453 Madison WI 53705
Telephone: 
(608) 263-3345
Focus Groups: 
Neuroscience/Neuropathology
Signal Transduction
Education: 
PhD, University of Nebraska Medical Center
Research Summary: 
The role of cell-cell and cell-matrix interactions in vascular development and neovascularization
Research Detail: 

Our laboratory studies the molecular and cellular mechanisms that regulate vascular development and angiogenesis. We are interested in how alterations in normal regulatory mechanisms, under pathological conditions such as ischemia, diabetes, and aging, lead to vascular dysfunction and growth of new blood vessels. We use the postnatal developing retinal vasculature as a model to study all aspects of vascular development. In addition, we have established a number of mouse models to study neovascularization associated with aging, ischemia and diabetes. Our in vivo studies are complemented with our unique ability to culture various retinal vascular cells from wild type and transgenic mice for in vitro mechanistic studies. The utilization of these models has provided novel insight into the role of a number of genes in regulation of vascular development and neovascularization. We are also utilizing our vascular cells for development of cell-based assays for identification of small molecules with potential therapeutic benefit for various diseases with a neovascular component. We are also working with our colleagues in the department of Biomedical Engineering on developing vascular mimetic three dimensional units using defined synthetic materials for high throughput screening of chemicals with adverse effects on vascular function, as well as identifying potential novel pro- and/or anti-angiogenic agentsĀ 

Selected Publications: 
Hydrogel Arrays and Choroidal Neovascularization Models for Evaluation of Angiogenic Activity of Vital Pulp Therapy Biomaterials.Saghiri MA, Asatourian A,Nguyen EH, Wang S, Sheibani N.J Endod. 2018 May;44(5):773-779. doi: 10.1016/j.joen.2018.02.008. Epub 2018 Mar 27.PMID: 29602530
Time-lapse microscopy of oxidative stress demonstrates metabolic sensitivity of retinal pericytes under high glucose condition.Ghanian Z, Mehrvar S,Jamali N, Sheibani N, Ranji M.J Biophotonics. 2018 Mar 25:e201700289. doi: 10.1002/jbio.201700289. [Epub ahead of print]PMID: 29577636
Mice dental pulp and periodontal ligament endothelial cells exhibit different proangiogenic properties.Saghiri MA, Asatourian A, Sorenson CM, Sheibani N.Tissue Cell. 2018 Feb;50:31-36. doi: 10.1016/j.tice.2017.11.004. Epub 2017 Dec 1.PMID: 29429515
Paclitaxel inhibited lysozyme fibrillation by increasing colloidal stability through formation of "off-pathway" oligomersKachooei E, Mozaffarian F, Khodagholi F, Sadeghi P, Karami L, Ghasemi A, Vahdat E, Saboury AA, Sheibani N, Moosavi-Movahedi AA.Int J Biol Macromol. 2018 May;111:870-879. doi: 10.1016/j.ijbiomac.2018.01.072. Epub 2018 Jan 17.PMID: 29352977
O-Linked Ī²-N-acetylglucosamine (O-GlcNAc) modification:a new pathway to decode pathogenesis of diabetic retinopathy.Gurel Z, Sheibani N.Clin Sci (Lond). 2018 Jan 19;132(2):185-198. doi: 10.1042/CS20171454. Print 2018 Jan 31. Review.PMID: 29352075
Vitamin D and regulation of vascular cell function.JamaliN, Sorenson CM, Sheibani N.Am J Physiol Heart Circ Physiol. 2018 Apr 1;314(4):H753-H765. doi: 10.1152/ajpheart.00319.2017. Epub 2017 Dec 22.PMID: 29351464
PEDF expression affects the oxidative and inflammatory state of choroidal endothelial cells.Farnoodian M, Sorenson CM, Sheibani N.Am J Physiol Cell Physiol. 2018 Apr 1;314(4):C456-C472. doi: 10.1152/ajpcell.00259.2017. Epub 2018 Jan 10.PMID: 29351407
Temporal diabetes-induced biochemical changes in distinctive layers of mouse retina.Aboualizadeh E, Sorenson CM, Schofield AJ, Unger M, Sheibani N, Hirschmugl CJ.Sci Rep. 2018 Jan 18;8(1):1096. doi: 10.1038/s41598-018-19425-8.PMID: 29348593
Vitamin D receptor expression is essential during retinal vascular development and attenuation of neovascularization by 1, 25(OH)2D3.Jamali N, Wang S, Darjatmoko SR, Sorenson CM, Sheibani N.PLoS One. 2017 Dec 22;12(12):e0190131. doi: 10.1371/journal.pone.0190131. eCollection 2017.PMID: 29272316
Versatile synthetic alternatives to Matrigel for vascular toxicity screening and stem cell expansion.Nguyen EH, Daly WT, Le NNT, Farnoodian M, Belair DG, Schwartz MP, Lebakken CS, Ananiev GE, Saghiri MA, Knudsen TB, Sheibani N, Murphy WL.Nat Biomed Eng. 2017;1. pii: 0096. doi: 10.1038/s41551-017-0096. Epub2017 Jul 11.PMID: 29104816