Youngsook Lee, PhD

Portrait of Youngsook Lee, PhD
Associate Professor
Cell and Regenerative Biology
4431 WIMR II
1111 Highland Avenue 4431
Madison, WI 53705
(608) 265-6352
Focus Groups: 
Signal Transduction
Research Summary: 
Transcriptional control of normal cardiovascular development and function
Research Detail: 

Malformation of the cardiovascular system is the most common form of human birth defect and cardiovascular disease is the leading cause of mortality. However, the molecular mechanisms that cause these heart diseases remain poorly understood.

The long-term goal of my laboratory is to advance our understanding of the transcriptional controls that guide cardiovascular differentiation / development and maintenance of normal cardiac function. The focus of our current research is to identify novel cellular and molecular mechanisms that lead to normal cardiac morphogenesis and function. For this, we are investigating molecular mechanisms that regulate cardiac-specific gene expression in vitro and role of transcription factors in cardiovascular development in vivo by generating transgenic mice. For example, we are studying molecular functions of the cardiac-restricted homeobox protein Nkx2.5, zinc finger protein GATA4, muscle-specific factor MEF2, and the nuclear factor Jumonji in regulating target genes and guiding normal cardiovascular development.

Embryonic stem cells are pluripotent cells. One goal of stem cell research is the development of specialized cells such as heart muscle cells. The directed differentiation of embryonic stem cells is then vital to the ultimate use of such cells in the development of new therapies. We are currently developing methods to isolate and enrich embryonic stem cell-derived cardiomyocytes.

Selected Publications: 
Pediatric Dilated Cardiomyopathy-Associated LRRC10 (Leucine-Rich Repeat-Containing 10) Variant Reveals LRRC10 as an Auxiliary Subunit of Cardiac L-Type Ca2+ Channels.Woon MT, Long PA, Reilly L, Evans JM, Keefe AM, Lea MR, Beglinger CJ, Balijepalli RC, Lee Y, Olson TM, Kamp TJ.J Am Heart Assoc. 2018 Feb 3;7(3). pii: e006428. doi: 10.1161/JAHA.117.006428.PMID: 29431102
Arabidopsis ABCG34 contributes to defense against necrotrophic pathogens by mediating the secretion of camalexin.KhareD, Choi H, Huh SU, Bassin B, Kim J, Martinoia E, Sohn KH, Paek KH, Lee Y.Proc Natl Acad Sci U S A. 2017 Jul 11;114(28):E5712-E5720. doi: 10.1073/pnas.1702259114. Epub 2017 Jun 26.PMID: 28652324
Late-stage differentiation of embryonic pancreatic β-cells requires Jarid2Cervantes S., Fontcuberta, M., Servitja, J.M., Fernandez-Ruiz, R., Garcia, A., Sanchez, L., Lee, Y., Gomis, R., and Gasa, R. Scientific Report, 7(1): 11643, 2017.doi: 10.1038/s41598-017-11691-2.
The Role of Leucine-Rich Repeat Containing Protein 10 (LRRC10) in Dilated Cardiomyopathy.Brody MJ, Lee Y.Front Physiol. 2016 Aug 3;7:337. doi: 10.3389/fphys.2016.00337. eCollection 2016. Review.PMID: 27536250
Cardiac defects, nuchal edema and abnormal lymphatic development are not associated with morphologicalchanges in the ductus venosus.Burger NB, Haak MC, Kok E, de Groot CJ, Shou W, Scambler PJ, Lee Y, ChoE, Christoffels VM, Bekker MN.Early Hum Dev. 2016 Oct;101:39-48. doi: 10.1016/j.earlhumdev.2016.05.017. Epub 2016 Jul 9.PMID: 27405055
LRRC10 is required to maintain cardiac function inresponse to pressure overload.Brody MJ, Feng L, Grimes AC, Hacker TA, Olson TM, Kamp TJ, Balijepalli RC, Lee Y.Am J Physiol Heart Circ Physiol. 2016 Jan 15;310(2):H269-78. doi: 10.1152/ajpheart.00717.2014. Epub 2015 Nov 25.PMID: 26608339
Increased nuchal translucency origins from abnormal lymphatic development and is independent of the presence of a cardiac defect.Burger NB, Bekker MN, Kok E, De Groot CJ, Martin JF, Shou W, Scambler PJ, Lee Y, Christoffels VM, Haak MC.Prenat Diagn. 2015 Dec;35(13):1278-86. doi: 10.1002/pd.4687. Epub 2015 Oct 30.PMID: 26338284
Quantitative proteomics reveals differential regulation of protein expression in recipient myocardium after trilineage cardiovascular cell transplantation.Chang YH, Ye L, Cai W, Lee Y, Guner H,Lee Y, Kamp TJ, Zhang J, Ge Y.Proteomics. 2015 Aug;15(15):2560-7. doi: 10.1002/pmic.201500131.PMID: 26033914
Jarid2 is induced by TCR signalling andcontrols iNKT cell maturation.Pereira RM, Martinez GJ, Engel I, Cruz-Guilloty F, Barboza BA, Tsagaratou A, Lio CW, Berg LJ, Lee Y, Kronenberg M, Bandukwala HS, Rao A.Nat Commun. 2014 Aug 8;5:4540. doi: 10.1038/ncomms5540.PMID: 25105474
Arabidopsis ABCG14 is essential for the root-to-shoot translocation of cytokinin.Ko D, Kang J, Kiba T, Park J, Kojima M, Do J, Kim KY, Kwon M, Endler A, Song WY, Martinoia E, Sakakibara H, Lee Y.Proc Natl Acad Sci U S A. 2014 May 13;111(19):7150-5. doi: 10.1073/pnas.1321519111. Epub 2014 Apr 28.PMID: 24778257
Lrrc10 is a novel cardiac-specific target gene of Nkx2-5 and GATA4.Brody MJ, Cho E, Mysliwiec MR, KimTG, Carlson CD, Lee KH, Lee Y.J Mol Cell Cardiol. 2013 Sep;62:237-46. doi: 10.1016/j.yjmcc.2013.05.020. Epub 2013 Jun 7.PMID: 23751912