Previous studies in our laboratory have provided evidence for paracrine Hh signaling in normal prostate development and human prostate cancer and shown that Hh signaling can accelerate xenograft tumor growth by a paracrine mechanism. We are now characterizing the target genes of paracrine activation and examining how the stromal phenotype determines the cassette of target genes expressed and the overall effect on epithelial proliferation and tumor growth. Complementary studies are examining the role of autocrine signaling in normal development and cancer.
Wade Bushman, MD, PhD
600 Highland Avenue
Madison, WI 53792
MD, University of Chicago, Chicago, IL
PhD, Brown University, Providence, RI
Hedgehog signaling in prostate development and prostate cancer; role of inflammatory signaling in regenerative-repair, hyperplasia and neoplasia; Role of prostate inflammation in producing prostate obstruction and changes in bladder function
Yu M, Bushman W. Differential stage-dependent regulation of prostatic epithelial morphogenesis by Hedgehog signaling. Dev Biol. 2013 380(1):87-98.
Casavant BP, Berthier E, Theberge AB, Berthier J, Montanez-Sauri SI, Bischel LL, Brakke K, Hedman CJ, Bushman W, Keller NP, Beebe DJ. Suspended microfluidics. Proc Natl Acad Sci U S A. 2013 110(25):10111-6.
Boehm BJ, Colopy SA, Jerde TJ, Loftus CJ, Bushman W. Acute bacterial inflammation of the mouse prostate. Prostate. 2012 Feb; 72(3):307-17.
Domenech M, Bjerregaard R, Bushman W, Beebe DJ. Hedgehog signaling in myofibroblasts directly promotes prostate tumor cell growth. Integr Biol (Camb). 2012 Feb; 4(2):142-52.
Lipinski RJ, Bushman W. Identification of Hedgehog signaling inhibitors with relevant human exposure by small molecule screening. Toxicol In Vitro. 2010 Aug;24(5):1404-9
Yu M, Gipp J, Yoon JW, Iannaccone P, Walterhouse D, Bushman W. Sonic Hedgehog-responsive Genes in the Fetal Prostate. J Biol Chem. 2009 Feb 27;284(9):5620-9.
Jerde T and Bushman W. Interleukin- induced IFG signaling in prostate develpment and reactive hyperplasia. Science Signaling (Cover) 2009, Sep 1;2(86):1-11.
Shaw A, Gipp J, Bushman W. Embryonic Sonic Hedgehog Signaling Stimulates Prostate Tumor Growth Using Reactive Stroma. Oncogene 2009 17;28(50):4480-90
Cook, C, Vezina CM, Hicks SM, Shaw A, Yu M, Peterson RE, Bushman W. Noggin is Required for Normal Lobe Patterning and Ductal Budding in the Mouse Prostate. Developmental Biology 2007. 1;312(1):217-30.
Shi X, Gipp J, Bushman W. Anchorage-independent culture maintains prostate stem cells. Dev.Biol. 2007; 312:(1) 396-406.