Gopal Iyer, PhD

Assistant Professor
Human Oncology
3133 WIMR
1111 Highland Ave
Madison, WI, 53705
Focus Groups: 
Cancer Biology
Signal Transduction
M.S. University of Baroda, India; University of California, Berkeley
Postdoctoral research: University of California, Los Angeles
Research Summary: 
1. Genomics and Molecular Imaging of Lung-Brain Metastasis 2. Defining the functional significance of bromodomain containing proteins in pan-cancer studies.
Research Detail: 

My laboratory is interested in the molecular mechanisms of metastasis in lung cancer. Metastasis is a poorly characterized process in cancer and focus on dissecting the mechanism of invasion from the primary tumor to distant sites is key to prevent the onset of this event. We focus on reverse-engineering differentially expressed genes obtained from genomic sequencing data of matched normal-primary tumor and brain metastasis patients in 3D lung models. We have identified a gene signature for metastasis, which are currently being tested using live cell and small animal imaging. The eventual goal is to reverse the onset of metastasis. Furthermore, our efforts focus on identifying immune genes that are altered in primary lung-brain tumor metastasis. Towards this effort, we adopt RNA and DNA sequencing of tumors, live cell fluorescent imaging and bioinformatics in the lab.

We are also interested to understand the functional significance of bromodomain containing proteins (BRD2, BRD3 and BRD4) across all cancers. Towards this effort, we have perturbed, using small molecule inhibitors, various cancers and identified key genes that regulate tumorigenesis in Myc dependent and independent manner. We are currently building small molecule variants towards bromodomains in collaboration with Dana-Farber Institute and testing them in tumor mice models. In this project, we use chemical conjugation strategies, genomic sequencing and small animal PET imaging to understand the overall mechanism of bromodomain targeting of tumors.


Selected Publications: 
Mohamed Y Elsaid*., Ankita Shahi*., Albert R Wang, Dana C Baiu, Chunrong Li, Lauryn R Werner, Sorabh Singhal, Lance T Hall, Jamey Weichert, Eric A. Armstrong, Bryan P Bednarz, Paul M. Harari, Gopal Iyer, and Mario Otto (2018) 0897-ETR Enhanced Radiosensitivity in Solid Tumors using a Tumor-Selective Alkyl Phosphocholine Analog. Molecular Cancer Therapeutics In press
Iyer, G., Price. J., Bourgeois, S., Armstrong, E., Huang, S. and Harari, P.M. (2016) Insulin-like growth factor 1 receptor mediated tyrosine 845 phosphorylation of epidermal growth factor receptor in the presence of monoclonal antibody cetuximab. BMC Cancer 16(1); 928
Iyer, G., Wang. A.R., Brennan, S.R., Bourgeois, S., Armstrong. E., Shah, P. and Harari, P.M. (2017) Identification of stable housekeeping genes in response to ionizing radiation in cancer research. Sci Rep: 2017, 7; 43763
Iyer, G.,* Pinaud, F., Xu, J., Ebenstein, Y., Li, J., Chang, J., Dahan, M. and Weiss, S. (2011) Aromatic Aldehyde and Hydrazine Activated Peptide Coated Quantum Dots for Easy Bioconjugation and Live Cell Imaging Bioconjugate Chemistry 15; 22(6):1006 *- Corresponding author
Dertinger, T., Colyer, R., Iyer, G., Weiss, S. and Enderlein, J. (2009) Fast background-free 3D superresolution optical fluctuation imaging (SOFI) Proc Natl Acad Sci U S A 106 (52):22287
Pinaud, F,F., Michalet, X., Iyer, G., Margeat, E., Moore, H-P. and Weiss, S. (2009) Dynamic Partitioning of a GPI-Anchored Protein in Glycosphingolipid-Rich Microdomains Imaged by Single-Quantum Dot Tracking Traffic 10(6):691
Schipper, M.L., Iyer, G., Koh, A.L., Cheng, Z., Ebenstein, Y., Aharoni, A., Keren, S., Bentolila L.A., Li, J., Rao, J., Chen, X., Banin, U., Wu, A.M., Sinclair, R., Weiss, S. and Gambhir, S.S. (2009) Particle size surface coating and PEGylation influence the biodistribution of quantum dots in living mice Small 5(1):126
Iyer, G., Michalet, X., Chang, Y.P., Pinaud, F.F., Matyas, S.E., Payne, G. and Weiss, S. (2008) High Affinity scFv-Hapten Pair as a Tool for Quantum Dot Labeling and Tracking of Single Proteins in Live Cells Nano Lett 10; 8(12):4618
Iyer G, Pinaud F, Tsay J, Weiss S. (2007) Solubilization of quantum dots with a recombinant peptide from Escherichia coli Small 3(5):793
Sarkar, S.,* Iyer, G., * Wu, J. and Glass, N.L. (2002) Nonself recognition is mediated by HET-C heterocomplex formation during vegetative incompatibility EMBO J 16; 21(18): 4841 * co-first authors